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F4/80 antibody alexa

WebThese antibodies target F4/80 in Mouse, Human and Rat samples. Our F4/80 monoclonal and polyclonal antibodies are developed in Rat and Rabbit. These antibodies have been verified by Relative expression and Cell treatment to confirm specificity to F4/80. Find the F4/80 antibody that fits your needs. WebF4/80, also known as EMR1, is a 160 kDa extensively glycosylated protein in the EGF-TM7 family of adhesion molecules. It is primarily expressed on macrophages and dendritic cells in mouse but is restricted to eosinophils in human. F4/80 plays a role in the development of peripheral tolerance.

F4/80 Antibody (BM8) SCBT - Santa Cruz Biotechnology

WebTarget Information. F4/80 antigen is a 160 kDa cell surface glycoprotein that is a member of the EGF TM7 family of proteins and shares 68% overall amino acid identity with human … WebA Goat anti rabbit IgG (Alexa Fluor ® 488, ab150077) at 1/2000 was used as the secondary antibody. Cells were surface stained with isotype control ( ab172730 ) or ab254293. Then fixed with 2% PFA for 10min followed by intracellularly stained with anti-myc tag conjugated to Alexa Fluor ® 647. download driver canon pixma mx497 https://rdhconsultancy.com

F4/80 Antibody Mini Review The marker and the antibody

WebAlexa Fluor® 647 Anti-F4/80 antibody [EPR20548] (ab237332) Description: Alexa Fluor® 647 Rabbit monoclonal [EPR20548] to F4/80. Application: Flow Cyt. Reactivity: Mouse. Conjugate: Alexa Fluor® 647. Recombinant. Anti-F4/80 antibody [EPR20548] - BSA and Azide free (ab234530) Description: WebTarget Information. F4/80 antigen is a 160 kDa cell surface glycoprotein that is a member of the EGF TM7 family of proteins and shares 68% overall … WebF4/80 is a well-characterized and extensively referenced membrane protein and is the best known mature mouse macrophage and microglial marker. The anti-F4/80 antibody clone CI:A3-1 is the original monoclonal produced against the F4/80 antigen in Siamon Gordon’s laboratory at the University of Oxford (Gordon and Austyn, 1981). clarkson beechmont

Alexa Fluor™ 488 Rat Anti-Mouse F4/80 - BD Biosciences

Category:F4/80 Products: R&D Systems

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F4/80 antibody alexa

F4/80 Antibodies Bio-Rad

WebProduct Images for F4/80 Monoclonal Antibody (BM8), Alexa Fluor 488, eBioscience™ ... (Product # 53-4321-80) (left) or 0.25 µg of Anti-Mouse F4/80 Antigen Alexa Fluor® 488 (right). Cells in the large scatter population were used for analysis. For Research Use Only. Not for use in diagnostic procedures. Not for resale without express ... WebStaining of mouse resident peritoneal exudate cells with Anti-Mouse CD11b APC (Product # 17-0112-82) and 0.25 µg of Rat IgG2a K Isotype Control Alexa Fluor® 488 (Product # 53-4321-80) (left) or 0.25 µg of Anti-Mouse …

F4/80 antibody alexa

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WebThe fluorescence conjugate options also include two exclusive Alexa Fluor® tandems; ... A3-1 F4/80 antibody is available for use in functional studies. An anti-idiotypic CI:A31 antibody, clone 17867, is also available from Bio-Rad which is designed as an IHC control antibody for use in conjunction with clone Cl:A3-1. F4/80 antibody, HCA154 ... WebProduct Information. 29414-1-AP targets F4/80 in IHC, IF, ELISA applications and shows reactivity with mouse samples. EGF-like module containing, mucin-like, hormone receptor-like sequence 1. PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20°C.

WebProduct Description. This Cell Signaling Technology antibody is conjugated to Alexa Fluor ® 488 fluorescent dye under optimal conditions and tested in-house for direct immunofluorescent analysis in mouse tissue. This antibody conjugate is expected to exhibit the same species cross-reactivity as the unconjugated F4/80 (D4C8V) XP ® Rabbit mAb ... WebThe BM8 antibody reacts with the F4/80 antigen (also termed Ly-71 in mouse and EMR-1 in human), an ~160 kDa transmembrane glycoprotein belonging to the EGF-TM7 family of G-protein-coupled receptors. F4/80 is considered a marker of choice for the identification of mature tissue macrophages, being broadly but variably expressed by this cell type ...

WebF4/80, also known as EMR1, is a 160 kDa extensively glycosylated protein in the EGF-TM7 family of adhesion molecules. It is primarily expressed on macrophages and dendritic cells in mouse but is restricted to eosinophils in human. F4/80 plays a role in the development of peripheral tolerance. WebThe molecular structure of F4/80 contains an EGF-like extracellular domain and a seven transmembrane motif, with a theoretical molecular weight of 160 kDa (1). Depending on …

WebF4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on the majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and ...

WebBioLegend's Purified anti-mouse F4/80 is a Rat monoclonal antibody. It reacts with Mouse. This antibody has been shown to work in applications such as: Flow Cytometry, Immunohistochemistry, and Western Blot. ... Alexa-647-conjugated rat antimouse F4/80 (BioLegend Cat# 123101, RRID:AB_893504), mouse anti-Cre (Millipore Cat# MAB3120, … clarkson because of youWebTwo hours after injection, peritoneal exudates were collected and stained with an FITC-conjugated anti-F4/80 antibody and analyzed by flow cytometry. TAMRA positive and F4/80 negative cells were considered as unengulfed apoptotic cells which were counted using Sphero AccuCount particles (ACBP-50-10, Spherotech, Lake Forest, IL, USA). download driver canon tr4650WebAlexa Fluor 350 (4) Alexa Fluor 405 (4) Alexa Fluor 488 (4)----- All A - Z -----Alexa Fluor 350 (4 ) Alexa Fluor ... Mouse, C. elegans, and E. coli. Each F4/80 antibody is fully … download driver canon scanner lide 25WebPhosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. The antibody was purified by affinity chromatography and conjugated with Alexa Fluor® 647 under optimal … clarkson beer store hoursWebF4/80 is a 160 kD glycoprotein. It is characterized as a member of the epidermal growth factor (EGF)-transmembrane 7 (TM7) family. F4/80, also known as EMR1 or Ly71, has been widely used as a murine macrophage marker, which is expressed on the majority of tissue macrophages including peritoneal macrophages, macrophages in lung, gut, thymus and ... clarkson bexleyWebAfter 4 h of incubation, anti-F4/80-APC antibody was used to label BMDMs, and these cells were collected for flow cytometry analysis. ... For CLSM observation of CRT exposure and HMGB1 release, the Alexa Fluor 488 anti-Calericulin antibody (Abcam, USA) and Alexa Fluor 594 anti-HMGB1 antibody (Abcam, USA) were used to stain the treated B16-F10 ... download driver canon ts3350WebF4/80 is a well-characterized and extensively referenced membrane protein and is the best known mature mouse macrophage and microglial marker. The anti-F4/80 antibody clone CI:A3-1 is the original monoclonal produced against the F4/80 antigen in Siamon Gordon’s laboratory at the University of Oxford (Gordon and Austyn, 1981). Today, Bio-Rad ... download driver canon ts6350