Phis2-p53

Author: rpdj

August undefined, 2024

Webb27 apr. 2024 · The glycogen synthase 2 (Gys2) and p53 generates a novel negative feedback circuit in which p53 represses Gys2 expression whereas Gys2 can stabilize … Webb20 dec. 2024 · b, The effecter and reporter constructs were co-transformed into the yeast strain Y187. p53His2 + pGADT7-p53, positive control, pGADT7-Rec2 vector that encodes murine p53 fused with GAL4 AD; p53His2 + pGADT7-JrDof3, negative control, pHis2 reporter vector that contains the cis-acting DNA consensus sequence recognized by …

Addgene: Vector Database - pHIS-2

Webb14 aug. 2015 · zt028 酵母单杂交载体系统 pHis2 Clontech ... zt1220 pGBK-p53 . zt1221 pGC-silencer-U6-GFP . zt1222 pGIPZ . zt1223 pGL3-FOXO-reporter-luci . zt1224 pGL4.13 . zt1225 pGL4.18 . zt1226 pGL4.19 . zt1227 pGL4.74 . zt1228 pGL5luci . zt1229 ... Webb9 apr. 2024 · +pHIS2-p53 3 1.2 酵母转化方法 pGAD53m +pHIS2-p53 1. 从YPDA平板挑取Y187单菌落接种于YPDA液体培养基4 ml，30℃，225 rpm，振荡培养18-20 h（过 … imrich car presov

Y187-pHis2YeastOne-HybridMediaKit 使用说明书

Webb7 dec. 2024 · For Y1H assays, sequences including the MBS or ERE elements from the promoter of VvMYBPA1 or VvMYBPA2 were cloned into the pHis2 vector. Mutant MBS or ERE elements were used as a negative control. The ORF of VvMYB14 or VvERF104 was cloned into the pGADT7 vector. Webb使用phis2载体，将启动子序列构建在his3报告基因前面的多克隆位点上，对应的转录因子构建在ad载体上即可。使用y187菌株，它相对于ah109菌株在基因组上少了his的报告基 … http://www.bnbiotech.com/beinuobio-Products-11104093/ imrich gablech

Yeast one-hybrid system—Matchmaker Gold - Takara Bio

pHis2载体质粒图谱、序列、价格、抗性、大小等基本信息_生物风 …

http://www.bnbiotech.com/beinuobio-Products-11104093/ http://www.biofeng.com/zaiti/jiaomuzajiao/pHis2.html imrich garlátyWebbProbe 2 and Probe 4 sequences as wells mutant Probe 2M and Probe 4M sequences were cloned to pHIS2.1, which drives the expression of HIS3 . GBFs were cloned to pGADT7. Yeast cells were co-transformed with a combination of the indicated plasmids or empty vector and plated onto nonselective (−Leu/−Trp) and selective ... im rich not perfect

"Webb30 okt. 2024 · Vectors pHIS2, p53-HIS2 + pGAD-Rec-53 and pMutant-HIS2 + pGADT7-TcMYC were the blank, positive, and negative controls, respectively. Transformed yeast cells were dropped onto a selective medium containing synthetic dextrose (SD) without leucine, tryptophan, and histidine but with 10–50 mM 3-aminotriazole (SD/-Leu/-Trp/-His … " - Phis2-p53

Phis2-p53

Multi-scale modeling identifies the role of p53-Gys2 negative …

Webb24 jan. 2024 · The p53 fragment was from pGBKT7-p53 used for Y2H. ... The promoter cloned in pHIS2.1 is strong autoactivator and the yeast colonies transformed with this is growing even at 100mM 3-AT.

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http://baiyk.com.cn/ProDetail.aspx?ProID=56 WebbpBIND pACT-MyoD pBIND-Id pG5 luc pCMV-BD pCMV-AD pBD-p53 pBD-NF−κB pAD-SV40T pAD-TRAF pFR-luc pGBKT7 pGADT7 pCL1 pGBKT7-53 pGADT7-T pGBKT7-Lam pACT2 AD Y187 AH109 pSos pMyr pSos MAFB pHis2 pHisSi-1 p53blue pGAG424 pLaczi p53his pGAD53m pBridge Y2H Gold Yeast strain Y187 pIND

Webb5 apr. 2024 · The Y187 strain co-expressing pGADT7-LkARF7 and pHIS2-LkBBM1 and the strain co-expressing pGADT7-LkARF19 and pHIS2-LkBBM1 grew normally on TDO medium containing 75 mM 3-AT, ... The bait vectors pGBKT7-p53 and pGBKT7-LaminC were co-transformed into AH109 with the prey vector pGADT7-LargeT as positive and negative … Webb1）准备质粒：构建pHis2-bait质粒→提取质粒； 2）制备Y187感受态细胞：YPDA平板活化→YPDA液体扩繁→制备酵母感受态细胞；或者直接购买Y187感受态细胞（CC306）； …

Webbp53-His2. Catalog No. PVT11251 Packing . p53-His2 Information. Function yeast Hybrid plasmid Resistance Kan Screen / Strain DH5alpha Culture temperature 37degrees … Webb酵母单杂交,p53-His,pLacZ,pHis，pHisi-1，. 产品型号：. 产品时间： 2015-11-08. 可提供近万种质粒载体、菌株、基因和细胞株,并可提供实验技术外包服务,如基因克隆、质粒构建、蛋白原核、真核表达及纯，pQE 载体，原核表达载体，克隆载体，毕赤酵母表达载体，毕赤 ...

Webb12 apr. 2024 · PHIS2-p53+pGAD53m SD-TL SD-TLH 阳性对照 3AT是酵母HIS3蛋白合成的竞争性抑制剂，用于抑制His3基因的泄漏表达。实验结果如下：图1：背景筛选结果结果 …

Webb9 apr. 2024 · 目的：发现与A相互作用的motif 方法：以构建到pGADT7载体上的A基因为诱饵，筛选酵母单杂交motif文库，经过对阳性克隆的基因检测、DNA测序和比对分析，确定与pGADT7-A相互作用的motif。材料： 1．诱饵克隆：pGADT7-A 2．诱饵载体：pGADT7 3．文库酵母质粒：motif 4．Clontech酵母单杂交系统 5．培养基 im rich national lampoon\\u0027s vegas vacationWebb更多详情咨询 www.bnbiotech.com, 021-61496710,15800960770 下面所有质粒和菌株，均为本公司实验室亲自验证过，可以提供技术支持，载体来源于promega,addgene,clontech,novagen,invitrogen可提供质粒图谱，可提供近万种质粒载体、菌株、基因和细胞株,并可提供实验技术外包服务,如基因克隆、质粒构建、蛋白原核、真 … lithium + oxygen balanced equationWebbEngineered Process im rich national lampoon\u0027s vegas vacationWebbBait pHIS2.1 containing repeats of your target sequence of interest Prey pGADT7-Rec2 vector containing a library gene Yeast Phenotypes His–, Leu–, or Trp– Requires histidine (His), leucine (Leu), or tryptophan (Trp) in the medium to grow; i.e., is auxotrophic for one (or more) of these specific nutrients Miscellaneous lithium oxide standardWebb2.制备感受态并将pHis2-DNA转化进入感受态细胞； 3.SD/-Trp平板检测转化结果。二、自激活检测 1.制备不同3-AT浓度梯度的SD/-His/-Trp平板，3-AT的浓度一般设定在50-100mM … im rich stupid richWebb18 juli 2006 · We generated yeast one-hybrid reporter plasmid by cloning the 356-bp p53 promoter fragment into the pHIS2 reporter plasmid (pHIS2-p53 prom.) and then cotransformed the yeast strain Y187 cells with pHIS2-p53-prom. reporter plasmid and pGAD-Rec2-p53 encoding p53-GAL4 AD fusion. We used pHIS2-p53 cons. reporter … i m rich gaither vocal bandWebbP0153 Mazda HO2S LF Circuit Malfunction. P0153 Mercedes-Benz O2 Sensor Circuit Slow Response Bank 2 Sensor 1. P0153 Mercury O2 Sensor Circuit Slow Response Bank 2 … im rich now songs