WebbProbe 2 and Probe 4 sequences as wells mutant Probe 2M and Probe 4M sequences were cloned to pHIS2.1, which drives the expression of HIS3 . GBFs were cloned to pGADT7. Yeast cells were co-transformed with a combination of the indicated plasmids or empty vector and plated onto nonselective (−Leu/−Trp) and selective ... http://www.qualityard.com/upload/2024-12-17/dbd8dacfb1f8d0626e1c10bc4840ba05.pdf
Horticulturae Free Full-Text Functional Characterization of Tea ...
Webb27 mars 2015 · In case of pHis2.1-Seq15 containing 4CRM-DJ1E, 50 m m 3-AT was sufficient to suppress background growth. As prey to select for TFs that bind to the … Webb1 mars 2024 · MdGSTF6-p-pHIS2 vectors were first grown on −Trp/−His medium containing 3-amino-1,2,4-triazole (3-AT) for screening. The concentration of 3-AT used was 100 mM. shangri la city china
PT3705-5 (PR32049) pHIS2
WebbPlasmid Phis2 1 Cloning Reporter Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article … Webb25 okt. 2016 · The 43-bp NWRE fragment amplified by PCR was inserted into pHIS2.1 (Clontech, CA, USA) to generate the plasmid pHIS2.1-NWRE as bait. The coding regions of IbbHLH3 and IbbHLH4 were separately cloned into pGADT7 as effectors. Lithium acetate-mediated transformation was performed to transform the constructs into yeast strain … WebbBait Vector Phis2 1, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more. Home > Search Results > TaKaRa > bait vector phis2. bait vector phis2 TaKaRa is a verified supplier TaKaRa ... shangrila club